Protection of Vacuum Systems Used in Tissue Culture Work Fact Sheet

When a laboratory vacuum is used to manipulate biohazardous materials, a suitable trap should be employed to insure that vacuum lines do not become contaminated. When a vacuum is used, the system should include an in-line HEPA filter as near as practical to each point of use or service cock. An approved reservoir and filtration apparatus for vacuum systems is described below: Vacuum filtration or aspirating supernatants into collection flasks are common laboratory procedures. During vacuum filtration or aspiration procedures, building and/or laboratory vacuum systems should be protected. A simple bench-top aerosol/fluid trap can protect building/laboratory vacuum systems. The basic vacuum trap consists of a disposable cartridge-type filter or equivalent installed in-line with a collection/overflow vacuum flask system.

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