Biosafety BioHazard Level 2 Practices

  • Keep lab doors closed. Access to lab is restricted when experiments involving BSL-2 material are in progress.
  • Post biohazard signs on door and equipment storing BSL-2 material.
  • Allow only persons informed and trained of the BSL-2 research to enter lab.
  • BSL-2 researchers must attend biological safety training.
  • All BSL-2 materials must be registered with the Institutional Biosafety Committee (IBC).
  • Wear lab coats only in lab (remove when leaving lab).
  • Face protection (goggles, mask, face shield or other splatter guard) is used for potential aerosol or sprays of infectious material when BSL-2 material must be manipulated outside of a biosafety cabinet.
  • Gloves must be worn when handling potentially infectious material.
  • Wash hands after removing gloves and before leaving the laboratory.
  • All waste is decontaminated by autoclave or other disinfection method before disposal.
  • Procedures are performed to minimize splashing and aerosol generation.
  • Biosafety cabinets are used when performing procedures likely to generate aerosols.
  • Work surfaces are decontaminated with 10% bleach or other broad spectrum disinfectant.
  • Centrifuges used outside of a biosafety cabinet must have a sealed rotor head or safety cup.
  • Biohazardous waste is contained in a leak proof container with a cover.
  • Decontaminate other contaminated materials before washing, reuse or disposal.
  • Sharps precautions. Broken glassware must not be dealt with directly by hand, but must be removed with a dust pan, tongs or forceps.
  • Biological Safety Manual defines special procedures and waste decontamination policies.